Terminator
Part:BBa_K4390070
Designed by: Zhongyi Liang Group: iGEM22_Edinburgh-UHAS_Ghana (2022-09-13)
This part is not compatible with BioBrick RFC10 assembly but is compatible with the iGEM Type IIS Part standard which is also accepted by iGEM.
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L1U1H08 Synthetic Terminator
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Secondary Structure
Measurement
- [http://openwetware.org/wiki/Cconboy:Terminator_Characterization/Results How these parts were measured]
Design
A transcription terminator designed for JUMP assembly (Valenzuela-Ortega & French, 2021).
Usage
This terminator was used in following composite parts.
| Name | Part Number |
|---|---|
| Untagged Dou-PETase | K4390088 |
| N-terminal Car9-tagged Dou-PETase | K4390085 |
| Untagged Tri-PETase | K4390089 |
| N-terminal L2NC-tagged Tri-PETase | K4390115 |
| N-terminal L2NC-linker-tagged Tri-PETase | K4390116 |
| N-terminal Car9-tagged Tri-PETase | K4390086 |
| C-terminal L2NC-tagged Tri-PETase | K4390082 |
| C-terminal L2NC-linker-tagged Tri-PETase | K4390083 |
| C-terminal Car9-tagged Tri-PETase | K4390081 |
| Untagged FAST-PETase | K4390090 |
| N-terminal L2NC-tagged FAST-PETase | K4390113 |
| N-terminal L2NC-linker-tagged FAST-PETase | K4390114 |
| N-terminal Car9-tagged FAST-PETase | K4390084 |
| C-terminal L2NC-tagged FAST-PETase | K4390076 |
| C-terminal L2NC-linker-tagged FAST-PETase | K4390077 |
| C-terminal Car9-tagged FAST-PETase | K4390075 |
| Untagged MHETase | K4390091 |
| N-terminal L2NC-tagged MHETase | K4390117 |
| N-terminal L2NC-linker-tagged MHETase | K4390118 |
| N-terminal Car9-tagged MHETase | K4390087 |
Reference
Marcos Valenzuela-Ortega, Christopher French, Joint universal modular plasmids (JUMP): a flexible vector platform for synthetic biology, Synthetic Biology, Volume 6, Issue 1, 2021, ysab003.
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Categories
Parameters
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